Large Animal Medicine Resident New Bolton Center Kennett Square, Pennsylvania, United States
Abstract:
Background: Blood transfusions are commonly performed in goats, but impact of donor blood storage on crossmatch compatibility testing is unknown. Hypothesis/
Objectives: To determine effect of whole blood storage on crossmatch results in caprine blood. Animals: 13 healthy adult goats
Methods: Whole blood (250 mL) from 10 goats was stored for 28 days. 90 major agglutination and hemolytic crossmatches using stored donor erythrocytes and fresh recipient serum were performed on days 0, 7, 14, 21, and 28. Grades ≥1 were considered incompatible. The effect of storage time on compatibility was assessed via mixed-effects logistic regression with individual crossmatch as random effect.
Results: On day 0, incompatibility was identified in 28/90 agglutination and 8/90 hemolytic crossmatches, compared to 3/90 agglutination and 25/90 hemolytic crossmatches on day 28. While there was no difference in agglutination incompatibility between days 7 and 0 (OR 0.77 [95%CI: 0.35, 1.7]; p=0.52), there was a lower odds of agglutination incompatibility on subsequent days (day 14 OR 0.10 [0.04, 0.27], p < 0.0001; day 21 OR 0.15 [0.06, 0.39], p < 0.0001; day 28 OR 0.05 [0.01, 0.14], p < 0.0001). There were increased odds of hemolytic incompatibility on all days compared to day 0 (day 7 OR 3.7 [95%CI: 1.52, 9.04], p=0.004; day 14 OR 6.87 [2.2, 21.37], p=0.001; day 21 OR 12.69 [3.89, 41.48], p < 0.0001; day 28 OR 11.68 [3.76, 36.31], p < 0.0001). Conclusions and Clinical Importance: Whole blood storage impacts crossmatch compatibility results in goats, markedly reducing agglutination and increasing hemolytic mismatch results.
